To acoustic stimuli. Similar to that done in mammals (i.e. in rats: ventral cochlear nucleus --> ventrolateral pons --> reticularis pontis caudis --> spinal interneurons, etc.).
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
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In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
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Analyzing the effectiveness of apply the Extended Producer Responsibility (EPR) concept for reducing pre-consumer apparel waste within a Star Fashion Pvt Ltd apparel company.
30 June 2024 3,764 0 View
Hello! I'm a PhD student and we need urgent technical help for the "Multimodal Pro Light Source 400W" equipment. We are having image acquisition problems in our laboratory. When we activate...
16 May 2024 9,545 0 View
Explain the concept of complexity theory Discuss the link between resilience and complexity theory Explain how these relate to public policy illustrate your discussion with appropriate example
05 April 2024 8,886 1 View
I've recently been trying to investigate the inhibition of H2S-synthesising enzymes: CBS, CSE and 3-MST, via PCR. We've ordered EGCG, PAG and i-3MT-3 to inhibit each enzyme respectively, and have...
16 March 2024 392 1 View
I performed virtual screening of some molecules in Auto Dock Vina and GOLD and obtained different results for the best ligand. I'm not sure if I did something wrong, or the docking program is not...
07 March 2024 3,680 3 View
I did a synthesis of a carbonyl ruthenium complex coordinated with the 1H-imidazole-1-ylacetic acid, but I'm having problems in the purification process. All the mixture is only slightly soluble...
03 March 2024 2,041 3 View
I want to test out some new primers, but I know from past PCRs that at least one of my reagents is contaminated. I'm on a time crunch to finish my project and am not sure if I can get new reagents...
21 February 2024 4,799 0 View
If primers amplify a specific region of DNA, and the same DNA is present throughout the body, can a single primer be used across different cell lines and different organs from the same species?
23 January 2024 6,465 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I need to install this program
25 July 2024 4,756 0 View
In general, when performing a Tafel analysis for corrosion resistance measurements, a line voltage scan is performed from say -250 to +250 mV vs. OCV at low scan speed around 0.125 mV/s. However,...
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I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
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