I am expressing CAT-intein fusions and somehow the c-term fragment is experiencing degradation. I have seen few papers that show the main product and some degradation products but don't give much details in the experimental section. I do get my splicing product but I would like to improve the stability of the c fragment. I am trying different things but I was wondering if you have any advice expressing/purifying intein c fusions or CAT proteins? Thank you!

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