Our flow cytometer, the BD FACSCALIBUR, seems to be malfunctioning: it won't stop sucking up the sample. Anyone have any experience with this problem/volunteer a solution. I was cleaned less than 1 week ago fully, and functioned perfectly twice before this problem.
this is common when somehow the software crash in the middle of a run. if this is not the case and you check that no pressure problems are present or your flask seals are ok, maybe you should enter this concern on Purdue flow cytometry forum to find some answers or directly consult BD
I had a similar situation before, but not exactly the same. It was: the machine won't stop even when I take off the sample tube, so the sheath start to flow back from the "sample incoming" pipe because of the air pressure from the pump. I finally realize that when you pull off the sample, two metal wire leave the solution or buffer, which makes the machine knows and then stop sucking the sample solution. So check the metal wire could solve.
Maybe can't help you, but good luck!
- Badges
- Science topic
- Similar topics
- Social Science
- Media
- Journalism