Reinold Goetz

3 Questions 6 Answers 0 Followers

Questions related from Reinold Goetz

Has anyone had any technical problems with their Flow Cytometer?

Our flow cytometer, the BD FACSCALIBUR, seems to be malfunctioning: it won't stop sucking up the sample. Anyone have any experience with this problem/volunteer a solution. I was cleaned less than...

19 November 2015 9,117 2 View

Any advice on adding external H2O2 to cells as a positive control for measuring ROS?

Hi, I was wondering if anyone has used H2O2 added externally to adherent cell cultures as a positive control to measure ROS production. What is the logic behind this (are you not just measuring...

21 October 2015 7,945 20 View

Does anyone have a good protocol for using DHE to measure ROS in adherent cells after pro-inflammatory stimulation?

I'm culturing adherent cells, stimulating them with a pro-inflammatory stimulus (LPS). I normally make a stock of 1mg/ml LPS and then use at a concentration of 10mcg/ml. I've tried various...

14 October 2015 9,796 10 View