Which packages can I use for meta-analysis of RNA-seq data and batch effect removal?

Dear All I have already collected RNA-seq expression profile of different studies issuing plant-microbe interaction. I have got read count files for different samples and genes. Now I want to...

14 January 2021 9,119 2 View

Qualitative research credibility?

One of the reviewers of my submitted manuscript asked about the study's formative evaluation. In my understanding, in a qualitative research credibility of the research is addressed by the...

14 April 2020 337 6 View

Dear all, I get one single band of 1000 bp using plasmid isolation kit, but I am confused about result since I didnt get 3 or 4 bands ??

Dear all, I am facing a problem with plasmid isolation from thermophilic bacteria. For one strain I was getting one single band of 1000 bp using Thermo Scientific GeneJET Plasmid Miniprep Kit and...

10 March 2019 3,162 14 View

How to achieve stable layer of zeolite particles for ATR analysis?

I am trying to get stable zeolite layer for ATR analysis. Until now I have prepared suspensions of the zeolite particles, added an aliqoute to the ATR cell and waited until it dried. But the layer...

14 February 2018 7,164 2 View

Can anyone suggest me a good price and technique laboratory where I can send some of my strains to carry out DNA-DNA hybridization

11 May 2017 926 1 View

IS there an example of a nonessential ideal that is not a z-ideal?

I'm searching for a counterexample for these sentence " every nonessential ideal in C(X) is not necessarily a z-ideal"

08 March 2015 1,128 6 View

Why are prodigy children suffering when they grow up?

Most of the prodigy children, so called "Wunderkinder", grow up to be normal people. How can they cope with diminished attentions and/or performance? What happens if the world won't revolve...

20 April 2014 7,151 12 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

What are the ranges of four diffterent poles of butterworth filter for ECG signal?

Hi, Can anyone please tell me what are the ranges of four different pole orders such as first order, second order, third order and fourth order respectively of butterworth filter for ECG...

23 February 2021 2,554 3 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Best synthesis method for large scale silver nanoparticle synthesis with high yield?.Best post processing steps after nanoparticle synthesis?

I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...

11 February 2021 4,964 13 View

Which anesthetic for cortisol determination in fish?

I am a bit confused. I am planning an experiment in the field where fish will be caught by electrofishing. Afterwards, they are supposed to be euthanised by using an anaesthetic. I found...

10 February 2021 1,101 4 View