I am planning my experiment to measure telomere length trough Q-FISH. Several papers have used TFL-telo to measure the intensity of the probe but I do not find the software very easy to use and the manual is very limited in information. I have tons of questions and some of them are: 1) How do you export the result of the fluorescence estimation in a format that can be analyzed (e.g. xls, csv). 2) When you have overlapping chromosomes how do you split them? I can cut one but then the other will be seen as two separate chromosomes. 3) How can you select part of a cromosome that the software is not including? I made the cut on crossed chromosome but now one of the two splits in not highlighted any longer. 4) Is there any tutorial available on the internet?
Hello, I am using this software and have the same questions with you.
Do you figure out how to use this software?
Thank you very much!
Thanks, me too.
And it's hard to find a software for telomere length measurement with FISH.
If you know how to use or find some good software to use, please let me know, thank you very much!
Hi, I found a software that works very well. It's a ImageJ plugin called telometer. Here the link: http://demarzolab.pathology.jhmi.edu/telometer/. Try it, it worked very well for me!
Thanks for your recommendation! I have just downloaded it, and it seems needing
username and password? I'm contacting the admin. I hope that I could get access to it. Anyway, thanks for your advice!
Thanks for the recommendation of Telometer! It is no problem downloading and installing the plugin, but how to get a username and password is really confusing since I have no idea who is "the system administrator“ and then how to get contact. Could you please tell me how to get the username and password? Alessandro Baldan Frankie Yu Thank you very much!
I also want to download the TFL-Telo software. However, I can't find any resource in the internet. could you tell me where I can get the software?
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