DNA FISH on testis is always showing good results , however the results for ovaries are poor, sometimes there is even no detectable signal at all. Is there any explanation?
I am conducting a study in which I have to measure the concentration of different hormones in cell supernatant samples by means of ELISA assays. Although the seeding conditions are always...
28 May 2023 7,886 1 View
According to the study titled as: Umbilical cord blood: Current uses for transfusion and regenerative medicine published by Nicoletta Orlando and her team is that the umbilical cord blood can be...
19 February 2023 7,370 1 View
I am working right now on my Ph.D. project and as a part of it need to create an ScFv antibody. I have found a Vh Vl sequence, but it was created from an amino acid sequence from a 1995 article....
09 February 2023 3,572 0 View
With good pictures?
26 December 2022 3,744 3 View
Hi, I have started my PhD in Lisbon and planning to create mABs using phage display technique. I did some research and didn't understood how can I create my library, of course I can go for the...
11 November 2021 1,831 4 View
Due to the COVID situation it is nearly impossible to find filter tips. Is it possible to do it without filter tips by making sure to clean the pipette with RNAse zap before use and working...
17 January 2021 9,121 3 View
I'm conducting a correlational study (Spearman rho) and there are no statistically significant relationships between the variables with a sample size of 37 (4 independent variables/1 dependent...
04 January 2021 6,168 5 View
Hello, I am analysing the cost benefit of doing: RNA extraction and library preparations myself Sending RNA samples for sequencing Sending the entire sample for extraction and RNA preparation...
26 November 2020 2,442 2 View
In my analitic laboratory we use a viscosimeter of Brookfield to have a measurements of viscosity, but now we have a Rheometer (MCR72) of Anton Paar, but if we measure the viscosity of a sample X,...
29 March 2020 2,421 7 View
Dear colleagues, I've been working on some lipid extraction analysis with QDa HPLC. I start with some biological samples that are spiked with a different final concentrations of a lipid mix...
14 March 2020 1,138 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View