Is there a specific pan-Nuclear antibody that works on mouse nuclei?

I'm looking for a pan-nuclear antibody for mouse. I've tried a LaminA/C antibody and it works, but there is differential expression amongst the cells in my tissue section. I'd like something that...

31 December 2017 7,594 2 View

How to determine whether a drug's effect is presynaptic or postsynaptic?

Rookie question here: In slice electrophysiology, if you're recording from a postsynaptic neuron and you perfuse a drug, how do you determine whether the effect of the drug is directly on the...

01 February 2017 8,218 4 View

Can site-directed mutagenesis and PCR cloning be performed on AAV vectors?

04 May 2016 9,352 5 View

Learning Calcium Imaging - What is a good introductory resource for learning how to perform calcium imaging?

I want to add calcium imaging (of dissociated neurons in a dish) to my repertoire of skills. I'm seeking a good introductory learning resource that has very practical info to help me execute my...

04 May 2016 3,575 3 View

Does Calmodulin bind M13 in the absence of calcium?

I am interested in the calmodulin interactions with its target peptides. One synthetic target peptide is M13, which is the basis for many GECIs such as GCaMP. My question is, in the...

04 May 2016 8,930 2 View

Do you test male and female mice at the same time in the Von Frey assay?

Hi all, I have a question about the von Frey assay (or really, any nociceptive assay where multiple animals are in close spatial proximity). I am using both male and female mice now, rather than...

04 May 2016 8,264 7 View

Separate pipettors for RNA work and PCR?

I'm beginning NGS library preps in my lab and I want to do everything correctly. My question now is about different sets of pipettors for different points in the workflow. We will have a dedicated...

02 March 2016 3,174 6 View

How do you properly (safely) dissect animal tissues that have been perfused with 4% paraformaldehyde solution?

It is a very common procedure to transcardially perfuse mice and rats with 4% PFA, and then dissect out the tissues. Formaldehyde (and PFA) is a known carcinogen, and all the safety material says...

01 February 2016 8,622 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View