It is very difficult to collect synovial fluid from smaller experimental animals. I would thus like to know a best method to collect it. Kindly share the real, worked out, experienced methodology to adopt.
Given the very small intra-articular volume of joints in mice (~2 uL), we don't even try to isolate synovial fluid. I have read a paper once where they claim to take the SF, but I am skeptical and would assume that any such procedure would run the risk of contamination by other fluids, such as blood or lymph. What do you need the SF for? We have started working with the air pouch model as the air pouch membrane is similar to synovium (composed of fibroblasts and macrophages) and you can easily extract fluid. One obvious downside is that the inflammation is induced by injecting an inflammatory stimulus (such as LPS or carrageenan) into the air pouch so it is more of an acute model of inflammation.
I also would be interested if other groups are able to reliably extract SF from mouse joints.
J
Thank you Dr.Jonathan
I focus on synovial fluid for macrophages and cytokine estimation. Can I get your paper on what you say air pouch model..Whether it could be developed as an arthritic model by any chance?
Thank you.
Our paper hasn't been published yet, but I did present this model at an EWWR workshop a few years ago. I have attached a copy of the presentation. It contains some good information and references.
Good luck,
John
our technique is published here
Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice.Seifer DR, Furman BD, Guilak F, Olson SA, Brooks SC 3rd, Kraus VB.Osteoarthritis Cartilage. 2008 Dec;16(12):1532-8. doi: 10.1016/j.joca.2008.04.013. Epub 2008 Jun 5.PMID: 18538588
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