So far I have tried 6H2.B4 (BD Biosciences) and 7H8.C2 (Novus Bio) - both appear only to result only in background fluorescence in porcine cells (SK6 and PK15 - I can get additional images showing the background if people think it will help), however 6H2.B4 stains perfectly in human cells (HEK 293T - see attached). I see no reason why the antibody should not cross-react with porcine cytochrome c as the porcine shares ~98% homology with rat protein (as the immunogen for 6H2.B4 was rat cytochrome c).

If others have encountered this issue, what antibody did you find to work?

Thanks in advance :) 

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