Has anybody tested any SBP antibody for detecting SBP-tag2?

20 January 2015 0 5K Report

I want to buy an antibody to detect SBP-tag2, however all the commercial antibodies were based on the 38aa SBP tag, there is no information whether any antibody can detect the 25aa SBP-tag2. And all the companies I have asked do not offer testing samples.

Badges
Science topic

More Yan Cui's questions See All

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Is CFI / TLI = 1 okay in Mplus?

Hi there, I did a CFA to 10 items on 2 latent variables. The model fit is too good and I've never seen it before. CFI / TLI = 1, RMSEA = 0. Is it okay, if everything else was done properly?...

24 July 2024 5,454 7 View

How to use differential equations to apply hybrid production in an inventory model ?

give suggestions

22 July 2024 5,664 2 View

Can I use soybean, sunflower, or olive oil to prepare a standard curve for lipid estimation in plants or pollen?

I am trying to analyze lipids in plants; specifically pollen by using Sulfo-Phospho-Vanillin (SPV) Method! Can I prepare a standard curve for this estimation using soybean, sunflower, or olive...

01 July 2024 3,267 1 View

Is PyMOL good for conformational editing of a protein structure?

I'm trying the edit the orientation of some side chains in a homology protein model I made. Can I use PyMOL for such tasks? If so, could anyone navigate me to some online tutorials? If not, what...

01 July 2024 4,409 4 View

How do I link researchgate with my pubmed publications?

I moved to a different institute and changed my email address. Somehow I lost the list of my publications. Is there a way to link to my PubMed publication list?

17 June 2024 5,312 2 View

How to replace Zeocin resistance gene in Pichia genome after integration?

I have been using Zeocin marker for target protein expression to verify integration in Pichia system. However, I would like to remove Zeocin marker since it's antibiotic related and not ideal for...

15 June 2024 3,851 2 View

How to estimate total sugars using lane and eynon method?

I want to estimate total sugars in carrot and products developed from it. I have been using lane and eynon method. I could get the values for reducing sugars but not for total sugars. What is the...

28 May 2024 9,382 4 View

Clarification on 3 x 2 factorial designs and reporting?

Hi, I am currently looking at a study where they used a 3 x 2 factorial design where they had 3 diets (A, B, C) and 2 enzyme supplementation levels (0 vs 1 %). Thus giving 6 treatments. Authors...

26 May 2024 4,626 1 View

Factorial Design Experiment Data in a Meta-Analysis?

Hi, I am currently creating a meta-analysis on the effect of growth performance of pigs in response to dietary fiber content in experimental diets as well as the influence of a fiber enzyme...

25 May 2024 8,855 1 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?

29 July 2024 7,714 4 View