Hello folks,
I'm interested in looking at ribosomes by negative-staining, and I understand that a lot of people use the FEI Morgagni for this purpose. However, the only TEM that I have access to is a Jeol 2000FXII. Is there anyone here that has used this EM for looking at ribosomes (or other macromolecular complexes?)
Best wishes,
Pramod
Any TEM will do.
I've even used a 300 kV Field Emission Gun TEM for a quick negative stain view of isolated biological macromolecular complexes (including ribosomes) before going to cryoTEM with the samples.
Sometimes you've got to use what you've got access to even though its a bit of overkill.
Cheers
B
Haha 300 kV FEG TEM for negative stain! Thanks for your quick response Brent really appreciate it.
Hi Pramod,
As Dr. Gowen said, any TEM is Ok. For negative staining the resolution is limited with sample preparation, not with microscope's properties. It would be better to use pure carbon film grids, not formvar.
The best way is to use a good cryo-TEM like FEI Titan Krios.
Good luck!
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