We have repeatedly observed practically no bioavailability of tryptanthrin in mice.
We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...
04 December 2020 7,657 6 View
We are assessing the activity of nanoparticle incorporated fabrics against bacteria and fungi.
24 November 2020 8,686 6 View
I am using KELPLUS ELITE-EX VA semi-automatic Kjeldahl distillation unit for nitrogen estimation. The salesman told me that unlike the older version; this version does not require making up the...
01 October 2020 844 3 View
We have designed an in house LAMP assay for the detection of a pathogen. We have used ThermoPol buffer, betaine, MgSO4, Bst polymerase and dNTPs in the reaction. I wanted to know why MgSO4 is used...
22 September 2020 6,916 5 View
So, I have been cloning a lot of genes over the years in various plant transformation and overexpression vectors and even though its a struggle always to generate positive clones, I usually am...
26 August 2020 8,364 9 View
I have an experiment in which I want to estimate the photosynthetic activity and carbon dioxide flux of wheat plant. However, in my institute does not have potable photosynthesis equipment. Is...
11 August 2020 9,701 2 View
As in Masters of Science in Agronomy in many places have only one year of research work, is it possible to convert it to a paper and publish it in an impact factored journal especially when the...
04 August 2020 4,252 6 View
How origami metamaterials can be modeled in ANSYS using shell element?
15 June 2020 9,331 1 View
After almost 13weeks of lock down to limit the spread of Covid-19 virus, Indian universities may reopen in the first week of July amidst the rising level of infections. This will require travels...
03 June 2020 1,467 7 View
Covid -19 pandemic situation has created a lot of ruckus in teaching institution. specifically if we talk about medical teaching institution, the magnitude of impact is many fold because our...
26 April 2020 9,008 8 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...
01 March 2021 6,474 3 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...
23 February 2021 7,429 1 View