We have repeatedly observed practically no bioavailability of tryptanthrin in mice.
Every concept feels like its predecessors stated it more precisely, with successors just adding context. It's rewording, but the understanding stays the same. For example: Structuralism, as...
10 June 2024 3,979 0 View
Skopos theory, developed by Hans Vermeer, is a functionalist approach to translation that emphasizes the purpose (Skopos) of a translation as the primary factor guiding the translation process....
02 June 2024 761 3 View
As a former PhD scholar, currently working as a Junior Research Fellow on a DST-funded project, I am seeking information on travel grants. A few months ago, while I was still pursuing my PhD, I...
03 February 2024 2,050 0 View
Trying to find the relationship between epigenetic and cancer more deeply.
30 December 2023 8,363 2 View
I used a python code to extract Lindemann index from dump files. But the problem is the code works fine with dump files with little amount of data and atoms. When I use large files if shows...
01 November 2023 4,002 1 View
Hello all , I have a two-plate configuration and there is a dielectric in between them . I launch electrons from plate and it reaches another plates through the dielectric . I kept the dielectric...
16 October 2023 5,702 0 View
Hello, I have performed MM-PBSA calculation of a protein-ligand complex. I utilised approx 750 frames (out of a total of 15000 frames) to compute the free enrgy change of binding. Then, I used a...
04 October 2023 247 2 View
primers are short oligonucleotides specifically designed for sequencing and PCR. But if we don't know where to start sequencing for a novel virus's genome then how the primers can be designed? or...
19 September 2023 4,636 3 View
Cancer means the uncontrol cell division, but what factors increase the risk of breast cancer in females other than the genetic issue?
18 September 2023 5,790 4 View
How can I pick up the microplastic particle when doing FTIR-ATR? Beacuse I can not directly place my filter paper under ATR.
01 September 2023 2,004 9 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View