How do you pool your PCR data from 3 independent experiments?

03 April 2017 847 3 View

How much time after siRNA transfection by Lipofectamine 2000 in INS-1 cells should I wait?

I'm optimizing my siRNA transfection with Lipofectamine 2000. I used 40-160 nM of Dharmacon siRNA and the ratio of Lipofectamine:siRNA is 10 ul for 10 pmol. I let the cells grow for 24h after...

02 March 2016 5,482 6 View

What happened to the RPMI-1640 media?

So recently my media has behaved very weirdly. Whenever I split the cells and transfer to a new flask containing orange media and put in the incubator, the media goes pink for several hours. And...

01 February 2016 6,684 4 View

How to do statistics in qPCR with multiple housekeeping genes and/or missing value?

So here is what I find reasonable to calculate the standard error: You calculate the propagated standard deviation s^2= s1^2 + s2^2, in which s1 and s2 are the standard deviation of housekeeping...

31 December 2015 9,191 3 View

What p62/SQSTM 1 antibody should I use?

06 July 2015 7,173 3 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to convert isotrophic Deriche filter into anistrophic?

12 February 2021 5,889 6 View