Has anybody ever worked with RGS2 antibody for Western blot?

02 February 2015 1 3K Report

Do you have any suggestions for the antibody/ies that really works? It shouldn't make bands on RGS2 -/-

Hi Nguyen and Lizzette.

I hope you will also share your experience with the antibodies on www.pAbmAbs.com? Here, scientists can share information about the antibodies they use in their research. Your input is therefore very valuable for other scientist when they are looking for reliable antibodies.

As a bonus a can let you know that all reviews received before dec 15th will enter a competition where the winner will receive €500 for personal spending. Every review counts as a lottery ticket-also reviews on antibodies that have already been reviewed by other scientists before you. It is quite simple to review. All you have to do is go to the website, click "submit review" and here it is described how to design the review.

Kind regards,

Simon

Badges
Science method

More Nguyen Truong's questions See All

How do you pool your PCR data from 3 independent experiments?

Provided that you repeat one experiment 3 times independently. For each experiment, you have 3 technical replicates, i.e 3 wells of the same treatment and calculated fold change of gene A from...

03 April 2017 961 3 View

How much time after siRNA transfection by Lipofectamine 2000 in INS-1 cells should I wait?

I'm optimizing my siRNA transfection with Lipofectamine 2000. I used 40-160 nM of Dharmacon siRNA and the ratio of Lipofectamine:siRNA is 10 ul for 10 pmol. I let the cells grow for 24h after...

02 March 2016 5,630 6 View

What happened to the RPMI-1640 media?

So recently my media has behaved very weirdly. Whenever I split the cells and transfer to a new flask containing orange media and put in the incubator, the media goes pink for several hours. And...

01 February 2016 6,790 4 View

How to do statistics in qPCR with multiple housekeeping genes and/or missing value?

So here is what I find reasonable to calculate the standard error: You calculate the propagated standard deviation s^2= s1^2 + s2^2, in which s1 and s2 are the standard deviation of housekeeping...

31 December 2015 9,299 3 View

What p62/SQSTM 1 antibody should I use?

I got double bands and non-specific bands when I tried p62/SQTSM1 antibody from a company. Has anyone have good experience with any other antibody to give me a suggestion?

06 July 2015 7,295 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

How to determine LOD values?

I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...

19 July 2024 1,086 1 View

Can any of you suggest how to find the detection limit.?

I am working on flourescence sensing of heavy metals using quantum dots.Can any of you suggest how to find the detection limit. Whether it is calculated from formula or we can get from instrument?

19 July 2024 7,411 3 View

Which is the best approach for anomaly detection in scanned image data set?

Anomaly detection in scanned image data set

18 July 2024 3,578 3 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View