Nguyen Truong

6 Questions 23 Answers 0 Followers

Questions related from Nguyen Truong

How do you pool your PCR data from 3 independent experiments?

Provided that you repeat one experiment 3 times independently. For each experiment, you have 3 technical replicates, i.e 3 wells of the same treatment and calculated fold change of gene A from...

04 April 2017 921 3 View

How much time after siRNA transfection by Lipofectamine 2000 in INS-1 cells should I wait?

I'm optimizing my siRNA transfection with Lipofectamine 2000. I used 40-160 nM of Dharmacon siRNA and the ratio of Lipofectamine:siRNA is 10 ul for 10 pmol. I let the cells grow for 24h after...

03 March 2016 5,584 6 View

What happened to the RPMI-1640 media?

So recently my media has behaved very weirdly. Whenever I split the cells and transfer to a new flask containing orange media and put in the incubator, the media goes pink for several hours. And...

02 February 2016 6,749 4 View

How to do statistics in qPCR with multiple housekeeping genes and/or missing value?

So here is what I find reasonable to calculate the standard error: You calculate the propagated standard deviation s^2= s1^2 + s2^2, in which s1 and s2 are the standard deviation of housekeeping...

01 January 2016 9,255 3 View

What p62/SQSTM 1 antibody should I use?

I got double bands and non-specific bands when I tried p62/SQTSM1 antibody from a company. Has anyone have good experience with any other antibody to give me a suggestion?

07 July 2015 7,243 3 View

Has anybody ever worked with RGS2 antibody for Western blot?

Do you have any suggestions for the antibody/ies that really works? It shouldn't make bands on RGS2 -/-

02 February 2015 2,820 1 View