Can an algorithm come into control and possession of data without the knowledge of or input from a human programmer?

Keeping in mind the adage on machine learning: data in, data out. I am curious whether there are neural network algorithms that could allow an algorithm to control and possess data on its own...

30 November 2020 860 11 View

Where was Fischerella sp. PCC 9339/Fischerella musciola (UTEX1829) isolated?

Dear colleagues, I am currently comparing some genomic features in different representatives of Fischerella genus isolated from different eco-types. Unfortunately, neither PCC nor UTEX collection...

11 July 2020 4,003 12 View

I'm obtaining some variable data from an intacellellular Ca2+ influx assay using Fura-2AM and require some troubleshooting help, can anyone assist?

-I am using Fura-2AM dye with probenecid to permiabilise TR146 oral epethelial cells. -flourescence is detected on a Flexstation. -Results from current experiments vary significaltly to previously...

29 March 2020 8,088 4 View

Tyramide Signal Amplification (TSA) staining on cell lines?

I am using Tyramide Signal Amplification (TSA) staining on tumor cells (human derived xenograft in mice) to stain tumors for a panel of six markers. I am interested in using this same assay to...

08 March 2020 6,929 3 View

There is a methodology to calculate dynamic level in oil wells?

Hi, somebody knows any methodology to calculate the dynamic liquid level of a oil well, without using downhole sensor or acoustic register. I appreciate your response.

28 February 2020 6,512 3 View

Can a research centre upload content published by its team of researchers?

I am wondering if the administrator in our research centre can upload published content for all researches housed within this group, or must authors upload their work individually?

01 February 2020 7,877 3 View

Is there any scientific evidence that there has been an overarching moral decline among (pre)teen students in the last, say, 10 years?

I hear this type of claim bandied about in certain education circles--namely, by k-12 practitioners themselves--that students' behavior, respectfulness, etc. has all but disappeared. I know that...

15 January 2020 455 2 View

Where can I find the parameters of Miltenyi gentleMACS protocols?

I'm planning to use a Miltenyi brain tumour dissociation kit, but without the gentleMACS machine. Does anyone know where I can find the parameters of the machine's programs/protocols?...

16 December 2019 6,150 1 View

What are the best cost-effective apoptosis assays?

My PhD student is working on phytochemical-induced cell death in cancer cell lines and we need to establish if the cell death is apoptosis or necrosis etc. As our budget is dwindling, does anyone...

18 September 2019 6,389 3 View

Using Guess=CARDS in the Gaussian QC software package?

Gaussian provides the ability to pass in custom molecular orbital coefficients using Guess=CARDS. However, it is not clear exactly where the coefficients should be placed. Are they placed in a...

03 July 2019 7,358 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View