Hallo CRISPR(S) :), which online algorithm is the best for designing gRNAs? specially in terms of determining the off-target effects in big genome?

More Ibrahim T. Hagag's questions See All

My question concerns MTT cell viability assay?

Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...

21 July 2024 9,914 1 View

Guidance needed for preparing the hydrogel samples for the XRD instrument?

I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...

20 July 2024 3,611 4 View

How can I extract the mathematical equation from existing Neural Network Model?

There exists a neural network model designed to predict a specific output, detailed in a published article. The model comprises 14 inputs, each normalized with minimum and maximum parameters...

14 July 2024 2,714 3 View

Why does my IVT gRNA product gives HMW smear in gel?

We are using MEGAshortscript™ T7 Transcription Kit for transcribing gRNAs from linearized plasmid templates. (MSP3485 digested with HindIII) (https://www.addgene.org/140082/) We let the reaction...

01 July 2024 6,342 0 View

How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?

Hello ResearchGate Community, How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?

26 June 2024 9,281 4 View

JCR 2024 Impact Factor Download File-Factors caused decline in IF are?

Why the IF of almost all journals declined so much? For the file download go to https://www.researchgate.net/post/JCR_Impact_Factor_2024-Factors_causes_decline_in_IF

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When use a HOMER Pro to design hybrid system PV/electrolyzer/hydrogen tank. How can I use all electrical load to power the electrolyzer?

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05 June 2024 5,609 0 View

Expert questionnaire, Is it possible for people with a specialty in music psychology to evaluate this questionnaire? Through the link below?

It is possible for people specialized in music psychology to evaluate this questionnaire Through the link below Thank you https://forms.gle/qzoD7jN2UDUhMjwQ7

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How can we extract DNA from yeast isolates without using any lysis buffer or lysis enzymes? Is there any manual protocol?

31 May 2024 548 4 View

Please could colect all my publications under my name ?

please could collect all my publications under my name ?

30 May 2024 2,891 1 View

How to learn more about SPSS and its Application?

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11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

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11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

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11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

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10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

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10 August 2024 7,429 2 View

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RNA Extraction Using Hot Borate Method No Longer Working?

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

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07 August 2024 6,622 1 View

Eden Ngah Den Low

I suggest you use multiple online tools, I tried four: Dharmacon, Genescript, Chopchop and invitrogen (not sure if those companies use the same database) and there are more, but eventually I found common sequences which were ranked high as having few off target in multiple tools.

Ibrahim T. Hagag

Thanks Eden!