Ibrahim T Hagag

5 Questions 41 Answers 0 Followers

Questions related from Ibrahim T Hagag

Hallo CRISPR(S) :), which online algorithm is the best for designing gRNAs? specially in terms of determining the off-target effects in big genome?

I have tried CHOPCHOP and E-CRISPR-TEST but they gave me different results in terms of genome off-targets for the same sequence! which online tool is the most trustworthy?

05 December 2018 7,859 2 View

How can you solve a problem of non specific bands at RT-PCR, you expect at 700 bp and you get clear band at 500 - can anybody help?

18 September 2013 4,158 20 View

Does anybody know what the mechanism of action of RLP lysis buffer of Qiagen kits is?

By which mechanism do they extract RNA, and why is RNA in this lysate very stable ?

27 August 2013 2,214 15 View

I want to blot AIV +ve allantoic fluid on WhatMan FTA filter paper. Can this be done with AIV specifically?

AIV allantoic fluid on WhatMan FTA cards.

21 April 2013 382 5 View

cDNA stability while shipping in RT for three days from one country to another?

I want to ship viral cDNA to the US for qRT-PCR that will take three days at room temperature. It will later be used for qRT-PCR. To what degree might it be degraded, or could it remain stable?

16 April 2013 10,025 18 View