I want to growth dental pulp stem cells in glass coverslips in order to induce differentiation into neurons. Months ago I used 5 ug/ml of poly-l-ornithine and 50 ug/ml of fibronectin as a matrix and cells growth very well. Now, using the same protocol, cells didn't attach and I don't know what happen.

I tried to modify the concentration of the matrix but the results are very similar: some cells reach the attachment but in a few days most of them die. Also, I used different "samples" of cells at different passages.

I'm very confused and I need some advice.

Thank you so much!

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