I recently bought a pre-modified e.coli bacterium containing pVIB plasmid.
The plasmid is resistant to ampicillin, but I wonder if there is a possibility to grow this bacterium without selective pressure. Maybe integrating the plasmid into the bacterial dna to prevent plasmid loss any ideas?
It sounds absurd. If you grow the strain containing plasmid without selection pressure, you may get recombinants, only strains without plasmid or other contaminants along with the colonies containing the plasmid. Then it will be very difficult to isolate the plasmid. The only way to conserve the plasmid in E.coli is to put selection pressure. Perhaps, you can grow the strain in broth containing ampicillin and isolate the plasmid from it for storage. More over, it is not a good idea to integrate the plasmid in bacterial dna, since it will be difficult to integrate and it will lead to the overall change in the bacterial genome and lot of other difficulties following.
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