Greetings, who knows where i can conduct a relatively cheap(pref in Nig) diff'ial gene expression analysis (usin microarray) on samples from lab mice?

More Uchechukwu Achor Obisike's questions See All

Good evening. Please what psychological theory/theories can be used to explain the concept of Moral Injury?

I am carrying out an undergraduate research and one of my independent variables is Moral Injury but I can't get a theory to explain this construct.

29 November 2022 4,665 3 View

Can I get a MATLAB simulink for a Single Phase Induction Motor?

A mathematically modeled one. Thanks.

23 November 2022 1,999 3 View

When using the Within-Subject Design, do I still require a control group since each subject would be acting as their own control this design?

I want to use the Within-Subject Design but I am confused as to whether or not the Control Group is still necessary. Please I really need help with this question.

23 April 2021 9,111 1 View

I need recommendations: Any free finance publication outlet with strong online presence?

finance and economic related journals

01 June 2020 1,935 0 View

Please i need help with explaining my '' messy '' sequencing result ?

Hi. i did a cloning reaction [ my insert is the gene for the PH domain ] using the Gibson Assembly cloning technique and used the products of the cloning reaction to...

07 September 2018 8,521 12 View

Do you encourage routine measurement of interpupillary distance (IPD) by a meter rule or a pupillometer, rather than solely depending on autorefr IPDs in eyecare practices?

We have been arbitrarily estimating interpupillary distance of patients for near IPD once a far IPD is obtained, either by an autorefr or any automatic device. This is often quite inaccurate and...

28 April 2013 4,249 20 View

Submit your article

Join us in shaping the future of #ClinicalNutrition research. This is a unique opportunity to showcase your latest research findings, in the field of clinical #nutrition. Submit your article...

01 January 1970 5,101 1 View

Structure-based Multiple Sequence Alignment Tools and Tools for predicting the domains on a query protein sequence.

Hi all, I am working on the PH domain of a mouse protein and i want to run a structure-based multiple sequence alignment to try to predict the boundaries of the PH domain and the predicted...

01 January 1970 5,859 5 View

BP monitoring among eye care providers

Why is BP monitoring not part of routine optometry services in many countries, despite the numerous evidence on the link between BP monitoring and glycaemia and eye diseases. Still one of my...

01 January 1970 3,230 5 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to get recent research on poultry production?

poultry production in nigeria

29 July 2024 5,518 2 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View