Hello fellow researchers,
I'm currently exploring the possibility of using Gram-positive bacteria for heterologous protein expression. My background primarily involves working with E. coli and yeast in the production of recombinant proteins, but I'm keen to expand my research into Gram-positive systems. Specifically, I'm looking into Lactococcus lactis and Bacillus subtilis, but I'm open to other suggestions.
Could anyone recommend a suitable Gram-positive bacterium for this purpose? Information about its ATCC number would be extremely helpful. Additionally, I'm interested in potential collaboration opportunities. My lab, based in Brazil, specializes in producing recombinant proteins using E. coli and yeast. We are open to partnerships that could facilitate our venture into the realm of gram-positive bacterial expression systems.
Any insights or advice on this transition, especially regarding the handling and optimization of gram-positive bacteria for protein expression, would be greatly appreciated.
Thank you in advance for your help!
Dear Caio Braganca
a suggest to you to test the Brevibacillus expression sistem
https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/brevibacillus-expression-system
i used in several years ago for expression of clostidium toxins
https://pubmed.ncbi.nlm.nih.gov/22747490/
https://pubmed.ncbi.nlm.nih.gov/23374160/
in parallel we tested also lactobacillus and Bacillus megaterium from Mobitec but i prefer Brevibacillus since B. megaterium is much more difficult to be transformed (you need to use protoplasts) and l.lactis due to the rapid media acidification do not reach high cell densites when you work in shake flask,
The main concern about Brevibacillus is that it smell quite a lot :-) but it is something that we can manage.
good luck
Manuele
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts