Does Grace' insect medium, unsupplemented prepared from powder suitable for sf9 suspension culture?
Thank you so much.
Hi, everyone, I am recently working on the baculovirus protein expression system. The bacmid I used is from our lab stock. There is no problem with the insert. Sf9 cells infected by a P1 virus for...
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Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...
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Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
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Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
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27 February 2021 3,904 2 View
We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...
24 February 2021 5,183 1 View
As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...
23 February 2021 3,175 3 View
This insect is minute in size around 1 mm and not clearly visible with naked eye.
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I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...
22 February 2021 10,099 5 View
My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...
22 February 2021 2,965 4 View