Good antibody against lysosomal enzymes, which works for immuno-EM.

More Roman Polishchuk's questions See All

How to make an apparatus for testing membrane permeability and selectivity?

Good afternoon everyone! I just can't find drawings or a description of the creation of an apparatus for determining the permeability and selectivity of membranes in relation to gases and gas...

14 January 2021 9,205 4 View

Fixation of whole rats organs in buffered formalin?

Dear colleagues, I would like to ask you if anyone has experience with the fixation of whole rat organs (heart, lungs, liver, kidneys, brain, testes) in 10% buffered formalin. Thank you, Roman

28 October 2020 3,175 3 View

How can you do biotynilation of antibodies in presence of gentamicin?

For some time we have used biotinylated antibodies for ELISA. Usually I use biotin N-hydroxysuccinimide ester to link biotin to antibodies (and I have used different carbodiimides for other...

28 July 2020 8,806 3 View

If you had to reference a publication which proves or at least rigorously argues that AI control problem is (un)solvable, what would you cite?

Links to such articles with some explanations would be great!

27 July 2020 7,035 6 View

What is the estimated projected price of electricity (or hydrogen) for bio-solar cells?

Hello, everyone! There are many reports about bio-based solar cells and their prospects in the scientific literature. All authors write that these devices will be low-cost. Does anyone know any...

13 July 2020 655 3 View

Can you help me?

I am looking for information as to whether some insects (or spiders?) similar in pattern of colouration (red anteriorly, blackish posteriorly, white transverse bands) to some Cleridae (Clerus,...

09 February 2020 4,416 3 View

Does somebody know about protocol of retroviruses and adeno-assosiated viruses?

Hello! I have a lot of practice with lentiviruses (shRNA, knock-in, CRISPR, etc.). And now I need to transduce human and mouse cells by retroviruses (knock-in). I recieved plasmid pQCXIP and in...

11 January 2020 1,245 3 View

Variation partitioning - Is there a all -encompassing function/package in R?

I am a bit frustrated looking for a competent function doing variation partitioning (e.g. on linear model output) it should be able to tick the following boxes A) calculate all overlaps and "pure"...

04 December 2019 9,273 3 View

Is there any problem with magnetite nanoparticles to be observed in a FESEM system?

I ask this because I had a problem with a FESEM FEI SCIOS system. According to the technician he made observations of my sample TiO2/MAGNETITE. After many observations and session of my samples...

25 November 2019 3,024 4 View

Has anyone had problems quantifying DNA using Qubit?

I use Qubit to quantify my DNA material, and I have never had quantification problems. However a few weeks ago my lab bought a new Qubit reactive which has a different color (slightly pink) in...

23 September 2019 3,943 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Jacques Gilloteaux

I am not familiar of one but one of my collaborators have done Cathepsins, i.e. Karen McGuire in Ohio (Cleveland). However I have no idea where she is as I am in UK and the lab she worked was dissolved in USA. Please try to ask Summa Health System in Akron Ohio for her contact

Roman Polishchuk

Thanks!!!