Hi.
I'm trying to evaluate a putative GO enrichment in a set of samples coming from tomato chromoplasts. To make an accurate study I would like to test if there's a GO enrichment in the proteome of wild type chromoplasts. In my set of samples there are more than 2000 protein sequences and that seems to be a problem for the geneontology website. Do you know how can I skip this issue?.
Thanks in advance
Lucio
Hi,
"seems to be a problem" hardly describe what the problem is.
From what you are describing, you have a list of protein sequences. I hope that is not the input data, for it would be quite big.
Do you have a list of Accession numbers?
What GO software/website do you use?
In general, not ALL of the protein you identified will be classified. There is a large amount of proteins which do not have a GO classification yet. Those are normally skipped by search engines.
Hi,
I used PANTHER website, and it doesnt say anything regarding why it isnt able to process my data. As well as you, I think that the actual problem is the that the number of accessions in the input is too big. Actually, if I take an small number of accession numbers then everything runs well.
Yes, I have the list of acccessions.
Thanks
Lucio
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