I decided to switch to glass pipettes from Hamilton syringe for AAV injections into the rodent’s brains. Now I am trying to find the best parameters for pulling the capillaries but cannot make the taper long enough. I need to break the taper in order to get 20-50 micrometers tip, the maximal length of such broken taper I managed to get is about 5.5 mm. Does anyone have experience with producing longer needles?

I am using Sutter P-87 puller and Drummond capillaries (ODxID=1.14x0.56).

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