Does anyone have any experience with glass bottom microplates (96-well)?
They will be highly beneficial for me for the cell based assay that I am working on but they are extremely expensive and I was wondering if they can be cleaned and re-used?
Dear Rose,
Of course, you can clean and reuse the glass bottom microplates. Look at the following steps:
1. Adhered cells can be removed by trypsinization.
2. Remove adsorbed media proteins by treating with 5% SDS solution.
3. Rinse the wells properly with PBS (2X) and with MiliQ water (2x) and dry.
Enjoy reusing it !!!
I hope it suffice your need.
Thank you Abshar!
I knew it had to be possible but couldn't find any protocols for reusing coated glass. As I intend to coat with poly d lysine. Thank you for your protocol!
I am not sure they can go through a sterilization cycle, since they use glue to stick the glass bottom to the plastic well part. Let me know if you manage!
Hi Anita!
If all heat sterilisation fails, I'm sure rinsing the plate in 70% ethanol (allowing to dry) and rinsing wells with MilliQ water will probably do the trick. (I am fortunate enough to have UV light in hood as well) but I have a few more methods to exhaust before trying these plates as it is a lot of effort in comparison to disposable plates.
Will let you know if i do attempt this!
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