The picture attached herewith is giemsa stain of mouse liver - i wish to know if these nuclei that are seen are that of macrophage or myelocyte -myeloblast or basophil or mast cell ?
I don't see any cell morphology. There is no way to tell.
I wish to know if this is correct protcol to study RNA from microbial colony lift blots ? - blotting microbial colony onto nylon membrane - baking 70c for 5 minutes - uv cross linking -...
01 February 2017 2,066 2 View
As Tsetse fly produces ultrasound after blood meal, could ultrasound serve as a factor for stimulating conversion of blood stream form of Trypanasomes to insect stage procylics and by aiding...
03 April 2016 3,027 3 View
In India, some citizens indulge in irresponsible activities like public smoking, spitting and getting drunk to disturb the public. One other issue in India is caste system that links one's birth...
09 October 2015 3,744 0 View
Poor people or any might be negligent, reluctant to record death in their family or might be discouraged to record proper reason for death if due to public health negligence. If the concept of...
08 September 2015 1,834 1 View
Can their luminescence eliminate baculovirus, as these viruses are light sensitive?
11 December 2014 372 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View