Have you pretreated the carbon cloth? A frecuent problem is due to the air trapped in the material, that avoid bacteria attachment. If the potential is referred to Ag/AgCl RE the potential seems to be too high. Typical values could be in the range between -0.4 and 0 V.
Thank you for your suggestion regarding anode material but anode potential is 0.6V in most of the research papers like prof. Lagon and team. Can you elaborate your comment for better understanding, if possible?
Regarding the question of the potential of the working electrode:
As you can see from this well-known bioelectrochemistry tutorial (http://www.xoch.info/uploads/7/7/9/2/7792995/ismet_tutorials_issue_2.pdf), the potential window for the study of the oxidation reaction in a bioanode does not exceed 0V vs Ag/AgCl RE. This fact does not indicate that anodes cannot work at these potentials, but it indicates that 0.6 is a less frequent potential.
As for the other question, my personal experience and that of other colleagues indicates that pre-treatment is often useful to avoid hydrophobicity problems (details inArticle Impact of the start-up process on the microbial communities ...