Does anybody know where CmR in the B. subtilis strain DB431 comes from (localization of cam gene) and is it 168 derivative or not? Thank you.
Regarding the genotype of DB431: nprE; aprE; epr; bpr; isp I; isp II
Reference:
- B. Jones, W.J. Quax
Alzheimer tau test and detergent cellulase: made by genetic engineering (No. 9 in a series of articles to promote a better understanding of the use of genetic engineering)
J. Biotechnol, 66 (1998), pp. 229–233
- Westers, Lidia, Helga Westers, and Wim J. Quax. "Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1694.1 (2004): 299-310.
About the CmR of DB431,
From this
"The following B. subtilis strains were used in this study: 168 (trpC2; protease-proficient wild-type); DB102 (hisA4, nprE; Kawamura and Doi 1984), DB104 (hisA4, nprE, aprE; Kawamura and Doi 1984), DB431 (trpC2, nprE, aprE, epr, mpr, bpf, isp-1) and WB600 (trpC2, nprE, aprE, epr, mpr, bpf, nprB; Wu etal. 1991). Plasmid pGS1 was based on pTZ12 (Aoki etal. 1987), encoded the B. subtilis nprE gene and conferred resistance to chloramphenicol. pGS1 was used to transform B. subtilis following the induction of natural competence (Bron 1990)."
- Stephenson, K., S. Bron, and C. R. Harwood. "Cellular lysis in Bacillus subtilis; the affect of multiple extracellular protease deficiencies." Letters in applied microbiology 29.2 (1999): 141-145.
The nprE gene in DB431 confer its resistance to chloramphenicol.
About the CmR of 168:
"A spontaneously occurring, noninducible, chloramphenicol-resistant mutant of Bacillus subtilis 168 has a mutation (cam-2) which maps in the ribosomal protein region of the chromosome near dal. Its presence does not confer dependence on chloramphenicol. Ribosomes of the cam-2 strain remained sensitive to chloramphenicol in in vitro protein synthesis. No chloramphenicol acetyltransferase activity could be detected."
It is mainly from cam-2 gene
Reference:
- Anderson, LINDA M., et al. "New chloramphenicol resistance locus in Bacillus subtilis." Journal of bacteriology 158.1 (1984): 386-388.
Dear Alex, thank you for your response. I went through all these articles, but didn't find the answer why DB431 that I bought from BGSC have CmR. Genotype they provide is: trpC2, nprE, aprE, epr, bpr, mpr, ispA on their site, but when the strain arrived Cm was added. Yes, you are probably right, this strain is probably BD431 with plasmid pGS1/nprE, which I can easily test; this also means that strain is no more nprE mutant as it has wt gene expressed from the plasmid. Also, it was probably constructed from 168 strain as they are both B. subtilis subsp. subtilis and trpC2. Thank you very much for your help.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology