Genomic extraction of Mycobacterium DNA from blood samples

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How to isolate and detect Avermectins from Streptomyces Avermilitis fermented broth?

I have a strain of Streptomyces avermitilis which produces a strong insecticide compound called amamectin (combination of avermectins), can anyone suggest it's isolation and detection procedure?

18 March 2013 251 1 View

Soil Borne Pathogens

From where I can have these soil pathogenic fungi like Rhizoctonia solani, Phytophthora and Pythium.

07 October 2012 3,179 6 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

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Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View

Hi. Can I get the list of publishers for microbiology-related short communications without publication fee (APC)?

I need to know which journals are exempt from publication fees (APC) in the area of microbiology

11 July 2024 6,346 2 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

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What are these aggregates in activated sludge flocs?

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Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

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12 June 2024 711 3 View

Rachid Lahlali

May be you should try Qiagean kit, it very easier the quality is good but the concentration seems to be lower as compared to chemical

Elizabeth E Traylor

I use a variation on the Qiagen DNA mini kit for extractions with blood serum, if that helps.

Kathirvel Maruthai

Hi, here U please find the attachment which we regularly used in our lab. We routinely process the extra pulmonary samples for PCR diagnosis of TB infection. So, I hope it will help U...

Rashmii Gwal

Thankyou sir for the valuable suggestion and procedure.. Can we use same protocol for body fluid also? such as Ascitic Fluid and CSF.