Kathirvel Maruthai

10 Questions 13 Answers 0 Followers

Questions related from Kathirvel Maruthai

How can I quantify (in %) DNA methylation level in CpG island by Methylation Specific PCR?

I am going to analyze DNA Methylation level in CpG sites and islands for certain candidate genes by Methylation Specific PCR. I have gone through the procedures but I am clear about designing...

12 June 2015 382 4 View

How can I carry out chromatin immunoprecipitation of histone modifications from blood?

Hello All,I am studying epigenetic modifications in children with reference to disease condition. I have to analyse three histone modifications at the same time in a single blood sample by...

16 July 2014 3,097 7 View

Can anyone suggest a procedure, and how to find out the inhibitory activity of a non-protein compound, against protein of interest?

I have a compound derived from a plant, and I want to look for its inhibitory activity against my protein of interest. It was already showed that the plant based compound is moderate in a toxic...

08 October 2013 2,139 1 View

Can someone advise on the genetic analysis of Treg and Tef cells?

I want to study the transcriptional changes in T cells in the context with TB infection. I have a doubt as whether flow cytometry or Flowcytometry with cell sorter will be the best one to do.

04 October 2013 3,907 2 View

How to find the primer binding sequence of a target gene?

I have a handful of primers, of which the details are unknown. In particular, I want to find the exact target sequence bases of the primers in their respective genes. So, I tried to find out, but...

06 December 2012 4,230 6 View

What is the differentiation between specificity and sensitivity when doing a method?

I am doing the series of molecular typing methods in the differentiation of clinical isolates especially, Mycobacterium tuberculosis. So, to differentiate each technique from others I am getting...

06 October 2012 9,867 11 View

Difficulties in RNA isolation?

I am going to start my protocols in RNA isolation, both qualitative and quantitative. What difficulties should I have in my mind? and what will be the solution for that?

03 September 2012 1,937 6 View

Will proteins get denatured in Ammonium Sulphate precipitation?

While doing extraction of proteins by using ammonium sulphate as salt system, and if it is left to be in salt does it affect the protein nature such as denature, activity lose, changes in...

03 September 2012 7,092 13 View

Purchasing flow cytometry and sorvall centrifuge instruments.

Our lab is about to purchase flow cytometry and sorvall centrifuge instruments, could anyone comment on these two instruments if they purchased them?

29 August 2012 8,391 9 View

How to inactivate the action of 5-azacytidine in cell culture to study further DNA methylation effect specifically after infection in same culture?

At first, I am planning to de-methylate (existing methylation) my culture with 5-azaycytidine drug and want to inactivate the further action of this drug. Then I am planning to infect my culture...

01 January 1970 3,019 1 View