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Questions related from Elizabeth E Traylor
I work in a veterinary pathology lab and we do immunohistochemistry staining by hand on all types of tissues that are FFPE. We have particular difficulty getting dermatopathology (skin) samples to...
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We are trying to find comprehensive datasets that include species, specific diseases, and location information. Does anybody have any good databases or can anybody send me monitoring data sets...
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I am wondering if anybody has opinions about using TAE Buffer vs the Buffer AE that comes with the Qiagen kit. We use the Qiagen DNA Stool Mini Kit to extract bacterial gDNA from stool samples,...
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I would love any suggestions you have for practical ways to avoid contaminating PCR reactions. What are some simple things to do? I know the basics, but I keep having sporadic contamination, so I...
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I am looking for some DNA to use as an internal standard when we do sequencing. I need to find a species that has never been found in the human gut, but has a medium GC content. If you know where...
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I am trying to amplify very small quantities of bacterial DNA using PCR. I currently use 40 cycles. I am having troubles getting my negative controls to be clean. I am working in a UV hood, UV...
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We are looking for a way to process a high number of stool samples. We currently use a Qiagen kit, which is labor intensive and can only extract 12 samples at a time. Do you know of any machines...
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