1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Why does the testosterone dissolved in dmso and methanol turns into a white dispersed precipitate when subjected to serum free media?

1mg of testosterone is dissolved in 20 microlitre of DMSO and Methanol respectively. The interpretation showed a positive result. However, when 1 ml of serum free media is subjected to the...

27 January 2021 8,213 1 View

After G actin polymerization and labelling I didn't got filaments. Why?

Purified G actin and got 42kda band and an additional band just below it. While performing G actin polymerization and labeling with rhodamine-phalloidin I didn't observe filaments under the...

20 January 2021 3,751 2 View

What all factors do we have to consider to interpret cytocompatibility of a nanomaterial from Actin DAPI stained images of HOS cells cultured with it?

Phalloidin stained filaments and dapi stained nucleus

08 December 2020 8,548 3 View

Will there be any changes in pcr result if one uses same dna with same PCR conditions and same pcr forward and revwerse primer after 10 months??

Actually, I am doing pcr for Kleb.pneumonia gene. I have did pcr of 90 samples initially and all were positive at that time. But now again when i am repeating the pcr for those 9 samples with same...

20 October 2020 4,383 11 View

How do different slots on patch helps in increasing the bandwidth of an antenna?

antenna designing

15 October 2020 1,788 4 View

What is the mathematical model of antipodal antenna design?

what is the mathematical expressions and equations used for the designing of antipodal structure of an antenna.

26 September 2020 7,948 3 View

Culturing of PANC-1 cell line?

I have ordered PANC -1 from Atcc. Upon plating them, the cells are taking around 4-5 days to reach 60% confluency. However, by end of 5th day , the background is becoming blurry with what seems...

15 March 2020 9,990 3 View

Can you consolidate a 5-point Likert Scale to a 3-point Likert scale for analysis?

Hi, I am a novice, first time researcher who started a research project with very little experience in designing questionnaires. My survey consists of 5 parts or sections with each section having...

19 February 2020 4,160 3 View

Is it possible to do CV, LSV studies in 3 electrode system for solid polymer electrolytes?

Can anyone help regarding ELECTROCHEMICAL STUDIES will be able to perform in 3electrode system for solid polymer electrolytes. As we are preparing solid polymer electrolytes combinations of two...

16 December 2019 7,271 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

Is there a recitation score bank system for fair evaluation and assessment of student recitation?

In actual and virtual class, even if there is rubric for recitation, students may still fail to receive fair grades or scores. Others are active in recitation and others are not or others may fail...

26 February 2021 8,387 3 View