PGE2 is a free acid and doesn't vaporize easily. You will have better luck using LC/MS instead. Alternatively using TMS-derivatization method may be an option if you must use GC/MS.

thank you for your help. The problem is that i have only a GC-MS equipment and am looking for an established protocol. Studies have been done with GC-MS but in in the methods described, they do not give a clear cut view . Do you have a TMS-derivatization protocol for PGE2

I haven't done PGE2 detection on GC-MS myself. But I found this article by Google search which sounds reasonable:

http://www.ent.iastate.edu/dept/research/physiology/pgs_taw.pdfhttp://www.ent.iastate.edu/dept/research/physiology/pgs_taw.pdf

You may want to give it a try.

Thank you

for any kind of volatile compound like prostaglandin whcih does not remain stable in GS should be done by Head-space GC and then MS

Do you have a protocol for that? Thanks

there are Instruments with attached headspace systems, also ull have to study a little harder and find out the best suited solvent for ur prostaglandin....which gives a very good gas:solid ratio...meaning the sample extraction is maximum

Hallo Mitradev,

once during my phd I was forst to analyse prostaglandine via GC-MS, it is for the most of them impossible! because you have to derivatizate them and withe 3 containing chemically active sites they get to heavy!

So try to find a cooparation partner, that would be more easy.

sorry