I am using 4%PFA Fix tissue, Embedded with O.C.T. 20u sections. After staining, the slides have a black/ grainy like residue that covers the entire slide front and back making them look dirty. During dehydration, I am able to remove the ones on the back with a Kimwipe, but the particles are all over the tissues. Is there anything that I can do to get cleaner slides?
Dear Matthew,
Thank you so much for your answer. I will follow your directions to the T and will let you know how it goes. On the same note, this is what might have happened to the developer solutions as well. Both Solutions turned milky with some precipitate upon addition of Tungstosilic acid. Each reagent was added consecutively.
Solution B: 1.9g Amonium Nitrate, 2.0g Silver Nitrate, 10g Tungstosilic Acid, in 1000mL of dH2O.
Solution C: 1.9g Amonium Nitrate, 2.0g Silver Nitrate, 10g Tungstosilic Acid, 7.6mL 37% F...in 1000mL f dH2O.
The precipitate might be Silver metal as well. Any thoughts?
Dear Gloria, I just would like to know whether your staining protocol is following the original paper by Gallyas 1971 [stain for neurofibrillary tangles] or is a work up after a general recipe as published in secondary (hand-book) literature (e. g. BANCROFT&GAMBLE, Theory & Practice of Histological Techniques, 2008/2011). Seconding what Matthew wrote (scrupulous cleanness of glassware, etc.) nevertheless there might be one or another step during your staining procedure producing theses masses of unintentional slver precipitates (for now I think about: using unfiltered or old conc. 37% FA-solution, contaminated A.[bi]dest., mixing the developer solution the right /the wrong way, any impurity of a chemical used in the process). Your informing about the processing steps might be of benefit for answering your request more precisely then. Regards, WM.
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