04 April 2014 1 2K Report

What are important areas of cancer that will become highlighted in future research efforts in Cancer?

More Michael Anthony Mannen's questions See All
Can anyone help with tissue homogenization of cardiac muscle for PCR work?

see above

02 March 2014 4,824 12 View

Is obesity as an autoimmune disease?

What do others think?

01 February 2014 8,431 18 View

Did Crick and Watson deserve the nobel prize for their participation in the discovery of the structure for DNA?

The discovering process and publication frenzy associated with discovering the structure of DNA was probably the most intense competition the biological sciences have ever seen. The competitors...

31 December 2013 4,690 9 View

Any reasonably intelligent scientist out there that does not believe in climate change?

If so, come forth and explain yourself oneself.

31 December 2013 5,052 21 View

Who is the greatest scientist ever? I vote Darwin

Who is the greatest scientist ever and why? Try to just pick 1. I know it is difficult to discern a great biologist from a great physicist. If you need to, pick the top scientist based on...

31 December 2013 1,802 52 View

Are you a quack scientist?

Over the course of my limited scientific career I have noticed a trend. A number of alarming posts and discussions inside and outside of RG have brought to my attention recent increased rates of...

31 December 2013 8,298 12 View

What to do about plagiarism in scientific research?

Plagiarism in science is dangerous. Scientific search requires a solid understanding of knowing of what is known and what has to be discovered. Plagiarism by other scientist can lead false...

31 December 2013 9,953 11 View

What are your thoughts on the supply and demand of science? Looking to get insight into the insecurity of getting high positions in research.

A hallmark of those promoting a career in research is to dramatize shortfalls in scientific labor. Promoters of this motif have come from as high up as Bill Gates, Chairman of Microsoft: "United...

31 December 2013 1,162 23 View

What are the best reference genes for cardiac muscle?

When doing real time q-PCR what are the best genes to used as references for normalization of data to account for experimental error?

31 December 2013 9,249 5 View

Tips on doing PCR

I am doing PCR work for the first time. Any tips/suggestions?

31 December 2013 2,834 5 View

Similar questions and discussions
Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close