Can anyone help with tissue homogenization of cardiac muscle for PCR work?

see above

02 March 2014 4,671 12 View

Is obesity as an autoimmune disease?

What do others think?

01 February 2014 8,270 18 View

Did Crick and Watson deserve the nobel prize for their participation in the discovery of the structure for DNA?

The discovering process and publication frenzy associated with discovering the structure of DNA was probably the most intense competition the biological sciences have ever seen. The competitors...

31 December 2013 4,493 9 View

Any reasonably intelligent scientist out there that does not believe in climate change?

If so, come forth and explain yourself oneself.

31 December 2013 4,891 21 View

Who is the greatest scientist ever? I vote Darwin

Who is the greatest scientist ever and why? Try to just pick 1. I know it is difficult to discern a great biologist from a great physicist. If you need to, pick the top scientist based on...

31 December 2013 1,632 52 View

Are you a quack scientist?

Over the course of my limited scientific career I have noticed a trend. A number of alarming posts and discussions inside and outside of RG have brought to my attention recent increased rates of...

31 December 2013 8,134 12 View

What are your thoughts on the supply and demand of science? Looking to get insight into the insecurity of getting high positions in research.

A hallmark of those promoting a career in research is to dramatize shortfalls in scientific labor. Promoters of this motif have come from as high up as Bill Gates, Chairman of Microsoft: "United...

31 December 2013 956 23 View

What to do about plagiarism in scientific research?

Plagiarism in science is dangerous. Scientific search requires a solid understanding of knowing of what is known and what has to be discovered. Plagiarism by other scientist can lead false...

31 December 2013 9,793 11 View

What are the best reference genes for cardiac muscle?

When doing real time q-PCR what are the best genes to used as references for normalization of data to account for experimental error?

31 December 2013 9,104 5 View

Tips on doing PCR

I am doing PCR work for the first time. Any tips/suggestions?

31 December 2013 2,659 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View