what is the simplest method to identify fusarium wilt in banana at entry level,
is there any instant method available?
Hi: If you want to identify the Fusarium spp on Lab level you should to grow tissue on PDA 25C for 3-5 day, you should see the spores under microscope.
For the field identify, wilt symptoms start from the old leaf with yellowish color.
http://www.fao.org/fileadmin/templates/banana/documents/Docs_Resources_2015/TR4/2-banana-fusarium-wilt.pdf
Dear Dr R Einstein Charles
Pls. find the attached files
https://www.omicsgroup.org/journals/diversity-and-distribution-of-the-banana-wilt-pathogen-fusarium-oxysporum-f-sp-cubense-in-china-2165-8056.1000111.php?aid=22205
http://www.publish.csiro.au/cp/AR9960637
http://www.promusa.org/Fusarium+wilt
http://www.ishs.org/ishs-article/897_46
http://www.cabi.org/isc/datasheet/24621
http://www.academia.edu/25191678/Molecular_and_Biochemical_Detection_of_Fusarium_Oxysporum_f.sp._cubense_as_the_Pathogen_of_Fusarium_Wilt_Disease_on_Banana_Musa_spp._
http://www.jnsci.org/files/html/2016/e239.htm
https://www.researchgate.net/publication/227617318_A_molecular_diagnostic_for_tropical_race_4_of_the_banana_fusarium_wilt_pathogen
Hoping this will be helpful
Regards
Prof. Houda Kawas
Article A molecular diagnostic for tropical race 4 of the banana fus...
Dear , Dr R Einstein Charles.
If you want to identify Fusarium you should apply the following steps:
1 - Bring portions from the infected plants to the laboratory .
2 - Cutting these plants to a small parts ( 1 - 2 ) cm.
3 - Washing the plant parts with tap water.
4 - Sterilize them with Sodium Hypochlorite for 2 minuets , then washing them with sterile distilled water.
5 - Putting them in Petridishes containing PDA and incubating them for 3 - 4 days at 25 oc.
6 - Preparing slides with lactophenol.
7 - Testing the macro and micro features by observe the colony shape , color and diameter .
8 - Noticing the phialides if they are mono or poly phialides.
9 - Noticing macro , micro and chlamydo conidia, if they are found , abundant , rare or absent. Also the microconidia in false heads or no.
10 - Measuring the macroconidia and noticing there shapes and the number of septa.
With my best regards
Dear , Dr R Einstein Charles.
You can also see the following items.
Best greetings
72
Hi Dr R Einstein Charles
You take samples (root, stem, and corm) from banana that shows wilt symptom as yellowing the old leaf, then 1) cut the samples small piece 1-2 cm, 2) washing and sterilize, 3) put on PDA media, 4) checking after 3 days, 5) do microscope slide and check the conidia and phialides (Must be monphialide of F. oxysporum)
Good Luck
Thank you Dr. R Einstein Charles for up-voting and I hope you have a good luck and more progress in your job.
With my best greetings
Thank you all for your answers. I need narrow technique to Identify Fusarium on field itself
Dear , Dr R Einstein Charles.
Fusarium oxysporum f.sp. cubense which caused wilting on banana distinguished by the following features :
1 - Colonies on PDA white , pale salmon or pale mauve or violet , often with salmon or orange sporodochia ; reverse pale salmon and mauve in the center, violet or greyesh.
2 - Macroconidia slightly curved with 3 septa with foot - shaped basal cells .
3 - Microconidia abundant , fusiform to kidney shaped , produced in false heads from short stout monophialides.
4 - Chlamydospores produced singly or in pairs.
Best regards
Dear , Dr R Einstein Charles.
The symptoms on the bananas in the field represented by the following :
1 - The external symptoms include chlorosis yellowing and death the oldest leaves.
2 - The internal symptoms include brown to red discoloration of the vascular system which range from discrete dots to large areas .
With best greetings
Dear
You may try the same as similar method we use
for Field evaluations, the symptoms on cutting longitudinal or horizontal of stem bases and incidental, variegated or dark brown color with or without scent enough to distinguish symptoms of fungal, bacterial and viruses . symptoms on the leaves and vegetative growth, distance between internodes in plants such as chickpeas and cotton.. other crops, and we usually used a degree for resistant or tolerance to differentiate between cultivars, also the patches distribution in the fields. but in banana I did not test them in the field .
Pls. find the attached files, look at page 117, in downloadPDF.pdf
Pls. find the attached files
https://www.daf.qld.gov.au/plants/field-crops-and-pastures/broadacre-field-crops/cotton/disease-management/fusarium-wilt
Hoping this will be helpful
Regards
Prof. Houda Kawas
Hi Dr. R Einstein Charles
You can know the type of isolates by pathogenicity test
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