Dear Mr. Bakir,

FTIR spectroscopy is able to answer these questions. The easiest way is to measure spectra of the pure components (HPAM and Xanthan gum separately) and compare them with spectra of your mixtures.

For example , the C=O stretching mode in carbonyl (and carboxyl) groups absorbs between 1800 and 1600 cm-1. In the case of H-bonding, an additional band at lower frequencies will appear.

Article Screening Effects in Solutions of a Hyperbranched, Dendrimer...

The N-H stretching modes of primary amines absorb between 3500 and 3100 cm-1. As for CO, in the case of H-bonding the peak of the bound moiety appears at frequencies lower than that of the free moiety. It might be that the absorbance strength is increased as well.

Article Dual-Frequency 2D IR Photon Echo of a Hydrogen Bond

The O-H stretching mode also absorbs in this spectral region, if the OH group is incorporated into H-bonds (ice band). Thus, sometimes people have problems to assign these modes correctly. However, O-H stretching apperas at slightly lower freuqencies than N-H stretching.

https://doi.org/10.1002/1521-3773(20020104)41:13.0.CO;2-U

Best regards.

Thank you for this. so my question is, can One still detect intermolecular interactions between polymers even in a brine solution? Thank you.

Yes, one can.

For films or melts I would prefer IR spectroscopy, for solutions (solvent and solute) I would try Raman spectroscopy, if the solvent absorbs to much of the IR signal.

Of course, you might also see interactions between your polymers and the solvent.

Article Hydrogen Bonding and Charge Transport in a Protic Polymerize...