From your point of view, Which is better putting more than one TEM-sample on a grid or one sample on each grid?
hello Dr Shabban;'
I prefer placing one sample on the grid at a time especially the crucial samples ,the reason for that is : if i have to go to that sample i know the orientation and I am not confused by the question of which sample is this??further more i want to get clear well focused image .
On the other hand i place more than one sample when i start my research examination of samples ie the first time i am examining the tissue it usually gives me a great idea about the tissue and some times i get very good results and usually i will snap as much pictures as i can ,knowing that if i have to come back to examine the samples it might be difficult to know where exactly i took this image from.also i use them for seeing some times what is the best way to orient cutting the sections.more over if you have many samples you might find that the image you want is masked by another specimen which is highly frustrating if the image is important .
hope that have helped
Sincerely
Dr.K.A.Galil.Professor of Dentistry
DDS.,D.Oral & Maxillofacial Surgery,PH.D,FAGD.,FADI.,Cert.Periodontist(Royal College of dental surgeons )
,Developer of The patented first App in Oral Histology for i Pad,i Phone ,i Pod Touch
visit the web site
https://itunes.apple.com/us/app/dgohe+/id1015383607?mt=8
and down load a free demo ,look at the bottom of the page .
see Dr.Gali llisted as #17 on 51 first researchers
http://www.uwo.ca/research/51_firsts/
it is better to use one sample no more, because when you are working with TEM, high energetic electron change your sample trough focusing on sample. some of your particle in sample melts and its TEM get damage.
It is better to place one sample at time. This way you know exactly what you're dealing with.
I have no idea what is on your picture, so no advice for your specimens.
When I work with biological thin sections I try to put as many as I can on a grid (usually 2-4). You may have overlapping of place of interest with grid, or some folds, or even holes... To have spares is not a bad idea.
Thank you so much for you all for your replies and opinions, they help very much.
Dr. Galil and Dr. Caserta agree with you mixing up samples is very frustrating. In this case one sample each is better.
Dr. Hossainian, if you are talking about observing nano powder (particles) or organic materials, I don't observe such materials.
Dr. Dusevich, The samples in the picture are for alumina scale (dark contrast) which is developed by oxidation of NiAl alloy (bright contrast) at 1000oC. I actually put more than one sample on one grid. For example if I have a set of 6-samples I put them all on one grid and another set of 6 samples on another grid as a backup. If I lost one I have another. I never mixed up samples. The problem of this style is, If the grid damaged or is dropped, all samples will be lost and sometimes you mix up. To overcome this problem I switched from Cu-grids to Mo-grids. They are more robust, but more expensive.
In my opinion, the adavntage of this style is you prepare your samples by FIB once then insert it to the TEM once. I think if you are lucky and didn't lose your grid, this technique will save your time.
- Badges
- Science topic
- Similar topics
- Biological Science
- Histology
- Sectioning