pANDA vector used for Agrobacterium bacterium mediated cloning.
Manu Kandpal If you could not find the sequence anywhere, it will be a good idea to drop an email and ask the Lab who constructed this vector.
Aim is to simulate the shape of Mobius strip (an elastic ribbon) in simple way ( using Abaqus Learning edition). In literature, Mobius strip is modeled as Kirchhoff Rod / Cosserat plate. But, such...
15 July 2024 4,388 1 View
In the context of acoustic wave propagation through heterogeneous media, PML is commonly employed technique to impose 'no reflection' boundary condition. I'm interested in knowing whether there...
19 February 2024 4,580 1 View
I am using photoshop for the same. Please suggest some good software for labelling SEM images like crater, holes etc
27 August 2023 3,878 3 View
please help me to have access to materials related to training and employee performance, that will facilitates my research proposal.
20 June 2023 1,148 2 View
related journals on training and organizational performance.
20 June 2023 616 3 View
or we can empty the cuvette with the previous sample and tap the cuvette on a paper towel and continue with replicate 2 and 3 and finally rinse when using sample 2. A curious question in case of...
09 April 2023 7,544 1 View
During molecular dynamics, I'm facing the error mentioned above. How can we fix it, I checked the lig.prm file to see if it is missing some angle parameters but it doesn't seem so, the file is...
02 November 2022 2,408 1 View
While docking SiO2 with a receptor protein in Autodock vina, the software threw an error which says "Si" is not a valid Autodock type. What is the possible reason and how to rectify this. How can...
17 October 2022 7,374 4 View
I would like to know the effect of oxygen vacancies on the optoelectronic property and photo conductivity of ZnO thin films and the reason for persistent photoconductivity in ZnO thin films...
26 August 2022 3,586 0 View
A protein which is selected using homology modelling, since there is no co-crystallized ligand attached to it. What is the procedure for the validation of docking procedure? Thanks in advance!...
24 August 2022 8,822 6 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
02 July 2024 5,448 2 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...
25 June 2024 1,197 0 View
I am working on a project that involves analyzing remote sensing (RS) images. I need to convert these images into vector data for further analysis. I am looking for automated methods or software...
22 June 2024 6,165 2 View
In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...
19 June 2024 7,822 3 View
Hi all, I have been facing problems with colony pcr for agrobacterium colonies. I did site directed mutagenesis, cloned to pcambia, sequenced and confirmed and then transformed to Agrobacterium....
02 June 2024 6,517 5 View