Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

What is special about Marketplace Simulations that make them ideal for online classes?

07 June 2020 3,212 9 View

You'r Opinion on Understanding the Voice of the Customer (VOC) through AI?

VOC is certainly nothing new. The more brands understand their customers, the better they are able to create products and services that meet their needs, gain competitive intelligence, and...

25 May 2020 6,220 6 View

Do you think privacy issues in social media is a major concern for consumers?

As we all know we share lot of information in social media, which is accessible by anybody around the world. Do you really think this is a threat to the consumers? Do consumers try to protect...

18 May 2020 3,900 18 View

Regression analysis for likert scale dependent variable with ordinal dependent variables , how ?

my survey responses are on likert scale whereas independent variables are ordinal. How to do further regression analysis in SPSS.

08 January 2020 6,781 4 View

How to edit the single phase UDF for a multiphase case?

In the first case (single phase case) liquid enters the tube and absorbs heat energy which is produced by the solid region. There is no phase change happening here. The following UDF is used to...

07 January 2020 5,829 1 View

Flow cytometry protocol for pERK staining?

Can somebody send me a protocol for intracellular staining of pERK in spleen and tumor cells?

22 October 2019 854 1 View

What are the other examinations which can be conducted for mml wear other that sem image. ?

I have sem image for mml wear but I am asked to give more examination on MML wear other than SEM images . Pls suggest the answer for the above . Thanks

10 August 2019 8,172 1 View

How do you get a first class or A grade in a research paper?

Can somebody provide me an example of a: -sophisticated -critical -logical, insightful and cogent research paper that is graded or known as a first class essay paper! I need some help ASAP-...

31 December 2018 6,460 0 View

How can I find Point Spread Function and Modulation Transfer function of multipinhole SPECT?

Hi, I am trying to find PSF(point spread function) and MTF(modulation transfer function) of multipinhole SPECT. Pinholes are arranged in such a way that, there is no overlapping between...

19 June 2018 5,942 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Why cloning of gene into pCAMBIA1304 vector is unsuccessful?

I have tried to clone a specific gene into pCAMBIA1304 vector. But all the attempts failed. I used ligation kit from both Promega Corporation and Takara Bio. However, there was one time I used...

04 February 2021 525 1 View