From where can I procure colloidal carbon nanoparticles in India?

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What is the Standard Procedure for measuring Total Lipid concentration in Daphnia magna?

currently working on a project involving Daphnia magna and I am intertied in measuring their total lipid concertation. I was hoping to get some insights or advice on the standard procurers for...

23 May 2024 8,846 1 View

What is 1335 in acetyl value and why this value put here as constant?

what is 1335 in acetyl value and why this value put here as constant? what is 1335 in acetyl value and why this value put here as constant?

10 January 2024 8,357 0 View

How to decide the practical applicability of our prepared super-capacitor electrode material for specific application in portable electronics?

Hello, Researchgate community, I have one query regarding deciding whether our electrode material is suitable for practical energy storage applications. When we prepare and characterize...

22 October 2023 6,027 3 View

How to decide the concentration need to induce oxidative stress in isolated mitochondria of rat brain by using either rotenone & sodium nitroprusside?

I'm trying to induce oxidative stress by using rotenone and SNP sodium nitroprusside in isolated mitochondria of rat brain. This is to study GSH assay from control (Mitochondria) group in...

11 June 2023 6,247 2 View

How to calculate the GSH present in mitochondrial protein in umol/gm?

While doing GSH on rat mitochondria, I have calculated protein concentration by Bradford's assay (1.35mg in 2ml buffer). In case of GSH assay I got concentration of 0.23mM (GSH) in mitochondria....

03 May 2023 2,820 6 View

How to treat mitochondria in-vitro by antioxidant and ROS inducer (rotenone) to study lipid peroxidation and GSH Assay?

I have to treat mitochondria with rotenone in aiming to induce Reactive oxygen species (ROS) and by antioxidant prevents it's ROS generation. Therefore,by In-vitro model how can I take the volume...

07 February 2023 2,865 0 View

What is the Methodology for calibration curve of rotenone?

Rotenone is the insecticide used as ROS Inducer in mitochondria. In aiming that what will be the method for the preparation of calibration curve for rotenone?

07 February 2023 5,471 0 View

How to treat mitochondria in-vitro by antioxidant and ROS inducer (rotenone) to study lipid peroxidation and GSH Assay?

I have to treat mitochondria with rotenone in aiming to induce Reactive oxygen species (ROS) and by antioxidant prevents it's ROS generation. Therefore,by In-vitro model how can I take the volume...

07 February 2023 6,362 4 View

Is tag removal required from fusion protein?

I have a thioredoxin tag with my therapeutic protein. I have purified it using Ni-NTA. Next, I want to check it's activity by Elisa or cell proliferation assay. Is it necessary to remove the tag...

11 November 2021 6,284 10 View

How to achieve biotinylation of polyclonal antibodies?

I want to biotinylate polyclonal antibodies. The application of these polyclonal antibodies would be in the lateral flow assay. I am not looking for any commercial kit. Please help with the...

23 September 2021 8,241 5 View

How gold procurement?

Please note it

29 July 2024 4,920 3 View

How gold procurement?

https://www.cleanteqwater.com/resources/case-studies/gold-mine-australia/

29 July 2024 5,161 1 View

Is there a link between age of voters, political preference and personal savings and investments?

I am searching for papers that help me answer this question. Furthermore, I need studies that analyse the saving behaviour of polically polarized persons (far left and far right). e.g. do far...

26 July 2024 7,780 0 View

What is the harnessing rainwater harvesting for climate change adaptation and how is India trying to ensure rainwater harvesting?

20 July 2024 6,041 2 View

How high should the remuneration be for test subjects in psychological research projects in Germany?

I am working on a project proposal (DFG project in Germany). I have the problem that I want to calculate how much funds I have to request for my test subjects (e.g., for qualitative interview of...

18 July 2024 1,690 0 View

Phengaris rebeli on Picea abies?

Today I took this video in Southern Bavaria, Germany. Video: https://www.youtube.com/watch?v=cCy1eLM6aE0 A female Maculinea rebeli was continuously tapping the leaves of Picea abies with its...

15 July 2024 8,099 4 View

What was the impact of the reform movement on India and impact of land reforms on small and marginal farmers?

09 July 2024 9,781 0 View

Is anyone from India going to Canakkale to attend International Conferece on intelligent and fuzzy system (INFUS-2024)?

No explanation or detail is required to understand the question.

03 July 2024 3,958 0 View

Has India been largest exporter of virtual water in world & virtual water trade affect environment & relationship between virtual & a water footprint?

Has India been the largest exporter of virtual water in the world and virtual water trade affect the local environment and relationship between virtual water and a water footprint?

03 July 2024 4,529 7 View

Is Vacation Homes and Vehicle Rentals in India succesful?

Assessing the Feasibility of Vacation Homes and Vehicle Rentals in India In my article, "Assessing the Feasibility of Vacation Homes and Vehicle Rentals in India: Understanding Market Needs,...

03 July 2024 5,535 1 View