From Nicobar islands, what could this pretty translucent soft green algae be?

More Felix Bast's questions See All

Please what is the best solvent to dissolve heme?

I have tried NaOH and NH3 so far but can't dissolve well.Thanks for the assistance. I can be contact personally on this email:[email protected]

04 January 2021 5,528 2 View

PVDF membrane degradation?

Hi, I'm performing western blots on different samples and I'm facing a some problems after eletrotrasnfer with PVDF membranes. In general, during the incubations with the primary and secondary...

18 November 2020 6,412 7 View

What to do when you found a published paper that doesn't belong to you but has your name as the author?

I found a published paper online (attached here) that bears mine and colleague's names as authors but the paper doesn't belong to us. The work was claimed to be carried out in Kenya. We are in...

22 March 2020 4,860 7 View

Do you know a (validated) instrument for the assessment of state embarassment?

I've found quite a few scales for vicarious and personal embarassibility as a personality trait so far (i. e. Kelly & Jones, 1997; Maltby & Day, 2000; Uysal et al., 2014...). As I'd like...

24 February 2020 8,142 4 View

Are Peak Areas of different silylations comparable?

If I have an analyte, that has different silylations, like 2-TMS and 3-TMS, how can I compare the peak areas? Can I simply sum them up? What if theres a different ratio of 2-TMS/3-TMS in my...

03 December 2019 273 4 View

What is a good topic for Blockchain applications in IoT?

Hello everyone, at the moment I'm looking for a topic for my bachelor thesis. I was thinking about Blockchain and Smart Contracts for cyber-physical Systems, in order to use it for Smart Factory...

23 October 2019 3,694 5 View

How to carry out western blotting for a 233 kDa protein effectively?

Hi, I want to carry out western blotting for a 233 kDa protein. I was told to use a tris-acetate gel due to the size of the protein. However, I was wandering would I be able to detect the...

25 March 2019 6,308 4 View

How to set up Coupled Displacement Boundary Conditions in COMSOL?

Dear Community, I recently switched from ANSYS Mechanical to COMSOL Multiphysics. So far I was able to transfer my models to the new platform but I yet miss one feature of ANSYS in COMSOL. I hope...

29 January 2019 4,937 3 View

SpeedVac of DNA and salt concentrations: downstream effects?

I often use a speed vacuum concentrator to concentrate my DNA prior to restriction digests, DNA ligations, NGS library preparation or target captures. Usually, my DNA is eluted in 10 mM Tris-Cl...

13 November 2018 6,049 3 View

How do I calculate NDVI images with different thresholds using Sentinel-2 image?

I want to set different thresholds for calculating the NDVI images using Sentinel-2 for the purposes of fishing out river sediments in the Dry and Wet seasons. I am using the Semi-automatic...

05 November 2018 694 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Razy Hoffman

I think that it should be Boergensenia probably B. forbesii

José M. Rico

Looks pretty much a Valonia sp.

or a Boergesenia sp. Check for B. forbesii

John M. Huisman

Was it collected in the intertidal? If yes then surely Boergesenia forbesii.

J. Pal

It is Boergesenia forbesii.

Vaibhav A. Mantri

Boergensenia forbesii for sure.