Fluorophore proteins (eg GFP, YFP, RFP) expressed in bacterial expression system are active?

Hi everyone, I have to express different mammalian proteins tagged with different fluorophores (such as GFP, YFP and others) under bacterial expression system and to use these purified proteins...

03 April 2019 1,501 4 View

Anyone familiar with nucleolar localization of proteins?

How does the nucleolar proteins/ribosomal proteins find its way to the nucleolus. Like nuclear proteins have NLSs and NESs which are responsible for their import and export in the nucleus...

03 April 2015 5,168 2 View

Is anyone familiar with BrdU / BrUTP incorporation?

09 October 2014 5,532 7 View

How can I use 2 different antibodies (for two different antigens) from same origin in IFA?

Can you please suggest me on how to use 2 different antibodies (for two different antigens) but from same origin in IFA. Both the antibodies are from mice. I tried the staining sequentially for...

04 May 2014 4,477 13 View

Can someone please tell me what protocol I should use to re-isolate RNA after DNAse treatment?

I use Trizol method to isolate RNA from frozen PBMCs. Sometimes, I get 260/280 ratio of ~1.8 at which I get slight amplification in my no-RT control, indicative of genomic DNA contamination. I...

03 April 2014 9,093 15 View

For recombinant protein expression in E.coli is Shine-Dalgarno (RBS) sequence mandatory?

I am cloning a protein in a bacterial expression system, but I cannot use RBS given in the vector, so I need to add it to my forward primer. Is it absolutely necessary? What is the optimum length...

11 December 2013 4,413 12 View

Nuclear protein isolation?

I am isolating proteins from crude nucleus using high salt concentration( 300-500 mM NaCl). Basically, I have to get nuclear proteins which will be used for CO-IP. In protocols I tried- a) 0.2mM...

10 November 2013 5,481 5 View

Cell cycle..

hi everyone...i have to see the effect of certain chemical compound on the cell cycle..basically i have to see their effect on the check points,whether it is causing cell cycle arrest at any...

08 September 2011 8,016 1 View

Methods to study a competitive protein-protein Interactions.

What methods can be used to study a competitive interactions between two different proteins (eg. A & B) for a common binding motif on protein (C).

01 January 1970 9,615 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

No title

02 March 2021 3,060 3 View