i analyze caffeine by HPLC . I inject solution and get 32125411 area in software. my question is how software get 32125411.
It integrated the area under the peak.
The software uses Simpson's rule to integrate the signal from the detector, see links
https://terpconnect.umd.edu/~toh/spectrum/Integration.html#history
http://www.intmath.com/integration/6-simpsons-rule.php
The links are important for understanding signal obtained from LCMS. Thank you sir for posting the link.
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