Any expert on Calu-3 cell culture?

I just started working months ago with this peculiar cell line and I still cannot figure out the doubling time or passaging. Some times take 72h to see double amount of seeded cells, some times...

23 October 2020 6,151 2 View

Which primers should I use for WT TP53 (qPCR experiment)?

Hi everyone, I am a student fairly new to experimental research! I am looking to do qPCR on different sarcoma samples to confirm the presence of TP53 (17p13.1). Since I am not seeking the presence...

10 December 2018 3,810 5 View

What kind of materials can I use for sputtering?

I have available an equipment PVD for sputtering/evaporation (Intercovamex TE12), can I use wires, pellets or powder for sputtering or evaporation? Or how should be the material physical presentation?

02 March 2018 6,887 9 View

I'm working in draft paper to simulate Band Excitation PFM & analyzing results with Principal Component Analysis. Suggestions to publish simulations?

10 November 2016 5,315 0 View

Microorganisms' Yield coefficients database?

Does anyone know if there is a database of known yield coefficients of microbial growth on different substrates?. Lets say for example, of the yield coefficient of yeast growth on glucose. Thanks...

09 October 2016 5,382 1 View

Microorganisms' Yield coefficients database?

Does anyone know if there is a database of known yield coefficients of microbial growth on different substrates?. Lets say for example, of the yield coefficient of yeast growth on glucose. Thanks...

04 October 2016 3,391 1 View

Is it possible to couple two physics in Comsol?

My Comsol version have the followings modules: AC/DC (Electric currents and Electrostatic) and Structural mechanics ( Solid mechanics only), so I would like to build a ferroelectric capacitor or...

02 March 2016 2,449 3 View

What is the mean recovery of CD3 + cells with magnetic sorter MACS?

We have been using in our lab MACS for cell separation but the retrieval has been low. If we add 20 million PBMC, we only retrieve about 2-3 million.

08 September 2013 1,964 6 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

No title

01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View