What undesirable conditions can lead to increase of this ratio?
I suggest, to emphasize the 222/208 ratio not too much for that purpose.
This ratio is also a parameter for amount and type of a-helical structure.
You may also want to consider this (older) reference, where coiled coil structure is characterized by another CD parameter:
The CD of Two-Chain Coiled Coils: Experiments
on Tropomyosin and Tropomyosin Segments
in the Tyrosine / Disulfide Spectral Region
MARILYN EMERSON HOLTZER, SWATANTAR KUMAR,
ALFRED HOLTZER
Biopolymers, Vol. 28, 1597-1612 (1989)
Best regards,
Martin
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