For the perfect identification of fish the method of DNA barcoding is realistic?

More Abhay Shelke's questions See All

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MD MOE RMSD

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Tyler Chafin Popular answer

If you assume that:

all possible identifications have sequenced examples;

You can trust the a priori identifications of the researchers depositing all of those reference sequences;

Unsampled cryptic intraspecific diversity does not exist within your species that might confound your result, or is not discriminated by your marker of choice;

You can trust an arbitrary threshold to distinguish between "match" (i.e. same species) vs. "not a match" (i.e. not the same species), AND can apply this same criterion ubiquitously (e.g. ignoring rate heterogeneity across lineages);

There is no mitonuclear disequilibrium (hybrid individual may have mtDNA "barcode" of species A, with nuclear genome of species B), or otherwise discordance between your marker (=single locus ancestry) and the rest of the genome;

etc...

No it isn't perfect... But it does provide a hypothesis when species ID is not known a priori...

Lavinia Iancu

Maybe this article can help. - https://www.ncbi.nlm.nih.gov/pubmed/15726991

Mitochondrial cytochrome b DNA sequence variations: an approach to fish species identification in processed fish products. Pepe T, Trotta M, di Marco I, Cennamo P, Anastasio A, Cortesi ML. J Food Prot. 2005 Feb;68(2):421-5

Regards,

Jorge Barros-Velázquez

Hi Abhay

It is. Apart from cytb other mtDNA and nuclear regions can provide you with information about intra- and inter-specific variability.

The proteomics approach is developing very quickly, but the current information is still limited.

best regards

Tyler Chafin

Juan J Schmitter-Soto

In addition to what Tyler writes (and I subscribe), empirically it seems to me that marine fishes are often successfully identified with barcodes, linking eggs and larvae to adults, etc.

However, this is not always the case with freshwater fishes. I know of several perfectly good species that are not told apart by gene COI.