as the DDT is soluble in organic solvent and those solvent like ethanol, methanol it self are toxic for how can we test the toxicity of DDT. i am open to all.
You may try with 5% aqueous DMSO.
Yes, DMSO should work.
DMSO
Than you all but i have tried the 5% DMSO but could not success-ed in dissolving DDT any suggestions how can i dissolve DDT in DMSO
I add 0.035g DDT in 1ml of 5% DMSO have tried multiple time vortex also have tried to put it in water bath at 45 degree still no success...
Did you try the other way? Dissolving DDT in 100% DMSO, and later diluting this to achieve desired concentration.
Varun Ahuja Sir now trying this way ...
You can take very small amount for the stock in DMSO, and then aliquot from this to reach desired concentration using your cell culture media.
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