How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Want to ask about equivalence ratio?

09 February 2021 5,273 2 View

In Michaelis-Menten Kinetics, why K(subscript)On have units (mol^-1)(time^-1)?

where K(subscript)On is the rate of Enzyme (E) + Substrate (S) going to Enzyme-Substrate complex (ES). I think I understand why K(subscript)Off and K(subscript)catalysis have units of time^-1....

11 January 2021 649 8 View

Do chemical and physical properties influence on the size of a polymer particle?

I would like to know if the properties of a polymer impact on the size of the particle it forms. I'm working with different polyssacharides (alginic acid from different seaweed), which are...

29 December 2020 7,333 6 View

How to make solution with molar ratio 1:40?

Currently i'm working to develop encapsulation using ferritin, based on many journals they always wrote the molar ratio between drugs and ferritin, for example 1:40, how to know the molarity that...

22 December 2020 6,582 2 View

Having issues understanding the particles/mL to molarity conversion, I've seen many different way but non of them seem to match one an the other?

I have 68nm spherical Au NPs, which gave me a concentration of 1.22 x 10^9 particles/mL. How can I calculate the molarity without the need for TEM?

22 November 2020 8,850 3 View

What solvent can I use to prepare a higher concentration of 5-Bromouracil?

I am standardizing a UHPLC method for separation of thioguanine phosphate metabolites. For this, I want to use 5-Bromouracil (5-BU) as an internal standard, however I am unable to prepare solution...

15 November 2020 4,982 3 View

How storage capacity for clathrate hydrate is calculated?

Storage capacity of hydrate solely depends on molar volume of empty hydrate lattice because rest of the terms get canceled (even moles of gas consumed). However, if we take temperature constant,...

16 October 2020 720 2 View

Please suggest that the way of obtaining the concentration (in mol) from the gas mixture (N2=98%, Ethylene=1% and Butadiene=1%) is correct or not?

I have a gas mixture (N2=98%, Ethylene=1% and Butadiene=1%). I want to obtained the molar concentration of each components for 1mL volume of the gas mixture. For that purpose, I have used the...

29 September 2020 6,233 1 View

How can I make a HgSO4 1M solution?

Hi everyone I want to make a HgSO4 1 Molar solution for deposition (electroplating) in my electrode, but HgSO4 is not soluble in water! What can I do !? Because My other solutions are all in water.

14 September 2020 3,461 3 View